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M9480871.TXT
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1994-09-05
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Document 0871
DOCN M9480871
TI Induction of immune mediators by defined lipid fractions from
Mycobacterium avium serovar 8.
DT 9410
AU Barrow W; Davis T; Wright E; Labrousse V; Bachelet M; Rastogi N;
Institut Pasteur, Paris, France.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:190 (abstract no. U-102).
Unique Identifier : AIDSLINE ASM94/94313074
AB Because of increasing evidence that M. avium lipids can modulate host
responses, lipid fractions from M. avium serovar 8 were evaluated for
their ability to modulate the immune function of human peripheral blood
mononuclear cells. A total lipid fraction was obtained by the Folch
extraction procedure and processed by column chromatography to derive
individual fractions which were defined by the presence of
glycopeptidolipids (GPL) or related components, and the amount of total
carbohydrate and 6-deoxyhexose, as determined by the Dubois and Dische
method, respectively. Total lipid and lipid fractions were assessed for
their ability to induce prostaglandin E2 (PGE2) and tumor necrosis
factor-alpha (TNF-alpha) release and their ability to affect macrophage
function as determined by intracellular growth experiments using an
unrelated strain of M. avium. All fractions affected human monocyte
function, with the more apolar fractions having the most adverse effect.
Only the total lipid and GPL fractions were able to induce significant
release of TNF-alpha and PGE2. Effectiveness for lipid fractions to be
immunomodulatory appeared to be dependent upon the amount of
carbohydrate, particularly with regard to 6-deoxy-hexose content,
suggesting the importance of GPL and related components in the immune
response to M. avium.
DE Dinoprostone/BIOSYNTHESIS Human Lipids/*IMMUNOLOGY/ISOLATION & PURIF
Lymphocytes/*IMMUNOLOGY Macrophages/*IMMUNOLOGY/MICROBIOLOGY
Mycobacterium avium/CLASSIFICATION/GROWTH & DEVELOPMENT/ *IMMUNOLOGY
Serotyping Tumor Necrosis Factor/BIOSYNTHESIS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).